Methyl Trans-Cinnamate Lab Report - can
. Methyl Trans-Cinnamate Lab ReportUBC Theses and Dissertations
Specific experiments were carried out in three areas. First, we developed a comprehensive transcriptomic database to facilitate the discovery of novel structural and regulatory essential oil biosynthetic genes in lavender. Annotated sequences include full length transcripts for all previously reported genes involved in the MVA and MEP pathways of isoprenoid metabolism, all prenyltransferases involved in the isoprenoid biosynthesis, and all terpene synthase genes cloned from various lavender species.
Further, the database contains TFs, representing 2.
UBC Theses and Dissertations
The assay identified 96 proteins that interacted with one or both promoters. The constructs were separately transformed into tobacco Nicotiana benthamiana leaves, co-expressing individually a subset of ten representative transcription factors. Six TFs induced expression from both promoters, two activated LiCINS promoter alone, and two did not induce expression from either promoter. Our results, which have enhanced our understanding of EO biosynthesis in plants, will help improve EO yield and composition in lavender and other plants through plant breeding and biotechnology.]
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